One experimental way of dealing with dormant/waxy embryos, using extended WEC (E-WEC)

Here is the R.clinophylla embryo, after 8 days of extended water embryo culture (E-WEC):





Even with this poor quality pic, I can see some of the difference/transformation that has taken place during this “extended water embryo culture”…maybe I could call this type of WEC procedure, E-WEC??!

LOL!!

Under bright fluro lights, it is actually a pale lime green color, the waxiness is now really hard to pick out.

My hypothesis is that waxiness in embryos equates to strong dormancy, and non-waxiness (pearly white, and or green tones) means a relative lack of dormancy. This is a working hypothesis I have, it is not proven.

Anyways, based on these changes, I felt it was a good time to sow it.

So, I sowed it in perlite in the same glass+perlite cup with its other waxy/dormant sibling. The cling wrap was sprayed with water on its inside, and was secured with a tight elastic band. I will periodically open the system and re-spray the cling wrap and surface of the perlite if need be (eg. once daily, or whenever), to keep the upper zones/surface of the perlite constantly as humid as possible.

Its other sibling had undergone E-WEC for 7 days, and was sown yesterday. It had one half-broken cotyledon at the time of its extraction, so I felt it was best not to focus on it too much, as it was already compromised from that injury, on top of its high dormancy/waxy condition (but it is still alive last time I checked it in the perlite glass cup).

waxiness in embryos equates to strong dormancy, and non-waxiness (pearly white, and or green tones) means a relative lack of dormancy.

It does, indirectly.

I have found the degree of waxiness to be an indicator of the level of dehydration. Dehydrated embryos are relatively dormant while those which retain more moisture are more metabolically active. In fact, in order for an embryo to break dormancy it needs to be moist enough to degrade abscisic acid during stratification.

Yes, totally agree!!

It is also really interesting, but not really surprising, to find in the same cross/batch of extremely dry achenes, some embryos to be pearly white amongst the other waxy ones.

I think separating these out and dealing with them differently (eg. differnig doses/times of water immersion) might give better final germiantion %. That is my hope, anyways.

The sibling rotted, and the more dormant one pictured above browned up and looked dead… a bad idea?? Dunno, but “E-WEC” has worked once before in similar waxy types…prolly worth a try if you have no other better option available.