I need this text’s methodology.
MARSHALL, H. H., 1975. New genetic sources of peonin and a new combination of anthocyanins in Rosa. J. Amer. Soc. Hort. Sci. 100 (4): 336-338s
I don’t have this paper, but here’s a link to EurekaMag.com where they sell it for $29.90.
https://eurekamag.com/research/000/439/000439724.php
New genetic sources of peonin and a new combination of anthocyanins in Rosa [acicularis, Rosa arkansana, Rosa dulcissima, hybrids]. [1975]
New sources of the floral pigment peonin in Rosa were found in R. acicularis, R. arkansana and R. X dulcissima. Peonin was transferred from the native tetraploid R. arkansana to fertile tetraploid hybrids with floribunda and hybrid tea roses. Cyanin, peonin and pelargonin were found combined in 10 seedlings derived from these crosses, a combination not previously reported in Rosa. A separate pair of blue fluorescing spots was associated with each of these 3 anthocyanins.
This one appears to be much the same, and is available from the same source.
Hortscience 9(3 SEC 2): 276 (1974)
New genetic sources of peonin in rosa and a new combination of anthocyanins
Marshall, H.H.
Some other papers by Marshall involving peonin:
http://bulbnrose.x10.mx/Roses/breeding/MarshallArkansana1976.html
http://bulbnrose.x10.mx/Roses/breeding/Marshall1977/Marshall1977.html
http://bulbnrose.x10.mx/Roses/breeding/Marshall1980/Marshall1980.html
http://bulbnrose.x10.mx/Roses/breeding/MarshallPeonin1983.html
http://bulbnrose.x10.mx/Roses/breeding/Marshall1983/Marshall1983.html
As for his methodology, the paper by Marshall and Collicutt (1983) explained:
In our study at Morden, pigment extracts from about 3000 roses were analyzed from 1973 to 1981. These samples were taken from native species, hardy and nonhardy cultivars and a large number of seedlings. Analyses of the results from 45 families of seedlings has given some insight into the color relationships found in roses. Paper chromatography was used to separate pigments. Amounts of pigments after separation were rated in daylight. These ratings were compared to those rated under long wave ultra violet (UV) light where the pink fluorescence of peonin and the yellow fluorescence of pelargonin served to confirm their identification and rating. Light absorption curves for the three pigments from roses and from other sources were developed using a spectrophotometer as a further check.
I am looking to develop a dark rose that is pigmented with only cyanin so I need to know which spots on the chromatography paper correlate to which pigments. Then I will know I have a cyanin rose. I had the paper but unfortunately misplaced it in a move! johannes
Angew. Chem. Int. Ed. Engl. 30: 654-672 (1991)
The Chemistry of Rose Pigments
Conrad Hans Eugster and Edith Märki-Fische
https://rosebreeders.org/forum/posting.php?mode=reply&f=2&t=56824#preview
The authors list the following to get you started.
‘Mister Lincoln’ (Swim & Weeks, 1964) dark red 66 (little 67, 68)
Papa Meilland’ (Meilland, 1963) dark red 66
‘Better Times’ (Hill, 1934) cherry red 66 (+ ?)
‘Maria Callas’ (Meilland, 1965) dark pink 66 (little 67)
66 is cyanin = cyaniin 3,5-diglucoside
67 is [no name] = cyanidin 3,7-diglucoside very rare
68 is chrysathemum = cyanidin 3-monoglucoside
‘Veilchenblau’ is also all cyanin, but it is complexed, which changes the color.
Your roses are all very beautiful choices. But I work with diploids:
‘Victory Year’
‘Martin Frobisher’
I have already crossed an unknown seedling it stays redder than ‘Red Fru DH’ x ‘Martin Frobisher’. The pollen parent despite so many flaws has proven itself a parent (I wonder if my efforts are worthwhile). I lost my ‘Victory Year’ but hope to get pollen this spring. I have used it before.
People ask why diploid? It is what I started with. My first cross in1992 was Rosa rugosa alba x R. woodsii. And on it goes. Being in zone2 made a difference.
Mister Lincoln is a strange rose. The color pattern is actually the same as some Pernet types. Instead, they are variations of reds. The face is a different color than the reverse and face edge. Oklahoma is fairly self-red, except that exposure darkens it.
Lasting Love is also a strange one, that seems to often behave like a non-red bicolor in breeding. The reverse color seems to be the dominating factor in a lot of its progeny.
There is a lot more to “reds” that meets the eye, and crossing red x red may not get desired or expected results at all.
Ecstasy is another interesting example. It’s a cross between a coral rose,bred from a red-orange rose, and the product between scarlet roses. Yet, Ecstasy is not only lacking any visual evidence of pelargonidin, its a purple-red somewhere between the color of Big Purple and Ingrid Bergman.
The moral is that when you say, “I am looking to develop a dark rose that is pigmented with only cyanin,” it be be more complex than that and one should look at multiple angles, including 1 to 2 generations of ascendants, to see if the selections are right for their choices. Not that I am saying what someone has to do. Just words of wisdom for free 
http://bulbnrose.x10.mx/KKing/RosePigments/Mikanagi2000.html
According to this report, the Chinas carry chrysanthemin as well as cyanin. Some of these carry the “chameleon” gene that synthesizes chrysanthemin after the flower opens, darkening the color.
Rugosas carry peonin as well as cyanin. The darker shades tend to have more cyanin than peonin.
‘The Fairy’ is pink, but almost all cyanin, with just the tiniest bit of pelargonin.
Karl, does Mikanagi et al give absorption peaks? I don’t have any for the sophorosides or coumaroylglucosides and I’m wondering what the shading effects are relative to the glycosides I do have.
https://www.rosebreeders.org/amember/content/f/id/26
Also, I had two cultivars of R. chinensis spontanea from Quarryhill that clearly showed light-dependent synthesis of anthocyanins.
And when did Quarryhill become Sonoma?
I know one of the two versions of ‘Sanguinea’ (the shorter one) darkens considerably with sunlight exposure. I actually wanted to get it again to play with these traits, because it showed no blackspot in Oregon, but I was wary of the mail order places having the giant version.
I bought and grew this rose ‘Sanguinea’ from Beales. I had so much hope in it. it was a glorious red when the petals opened, stunning! But It blued and further it really resented Cassiorhodon pollen. I can understand why Svejda gave up that breeding line.
I bought and grew this rose ‘Sanguinea’ from Beales. I had so much hope in it. it was a glorious red when the petals opened, stunning! But It blued and further it really resented Cassiorhodon pollen. I can understand why Svejda gave up that breeding line. the tall or short one i wish I could tell you.
Don, Quarryhill Botanical Garden officially became Sonoma Botanical Garden on March 17, 2021, and they have a brief statement about the rebranding at the link below (in short, it seems that they had acquired a second property where they will focus on California native vegetation; maybe they felt that the name Quarryhill had become synonymous with Asian plants):
https://sonomabg.org/news.html
Stefan
Sophorosides shift towards orange, rutinosides toward magenta.
R. moyesii cv. Geranium contained [cyanidin 3-sophoroside] as 56% of total anthocyanins.
Cyanidin 3-rutinoside (magenta) and peonidin 3-rutinoside (pink)
I don’t know about the 3-ρ-coumaroylglucoside-5-glucosides of Cy and Pn.