hybridizing rose virus alert

Strawberry latent ringspot virus is one of the rose viruses that can be passed through the seed, see:

http://www.agdia.com/testing/cropscreens/rose.shtml

It has now been found to be widespread in California strawberries for the first time in the United States ( “The number of plants that tested positive as well as the geographic distribution of the virus indicates that the virus is widespread in California, but further testing is needed to identify its distribution in other states.”) see the following link

http://www.apsnet.org/pd/searchnotes/2004/0309-02N.asp

Note, this report does not discuss its distribution in roses in the U.S.; but being widespread in California strawberries, I feel that the chances are that it will appear in roses.


It also has been found for the first time in roses in India:

Title: Strawberry latent ringspot virus Infecting Roses in India.

Authors: Kulshrestha, V. Hallan, G. Raikhy, R. Ram, and A. A. Zaidi

Authors affiliation: Floriculture Division, IHBT (CSIR) P.O. Box 6, Palampur-176 061 (H.P.), India.

Published in: Plant Disease, volumn 88, page 86, (2004).

Abstract: "Rose is an economically important crop of India and the world. A survey of rose plantations in and near the Kangra Valley of Himachal Pradesh, India, showed virus-like symptoms, including yellow flecking in young leaves and reduction in leaflet size, while some were symptomless.

These symptoms are similar to those for Strawberry latent ringspot virus (SLRSV) (1). Sap inoculation from symptomatic and some symptomless leaves to Chenopodium amaranticolor resulted in chlorotic local lesions followed by systemic chlorosis. SLRSV was detected in this indicator host and six rose cultivars (Happiness, Iceberg, First Prize, Ganga, Pink Panther, and Oklahoma) showing characteristic symptoms of SLRSV using enzyme-linked immunosorbent assay (ELISA) with ELISA kit (DSMZ, Braunschweig, Germany). Reverse transcription-polymerase chain reaction was performed with SLRSV-specific primers (2), and a product of the expected size of ~181 bp was amplified. The authenticity of the fragment was confirmed by sequencing. Isolated SLRSV was also inoculated to seed-grown rose seedlings and after 20 days postinoculation the same symptoms (yellow flecking in young leaves) were observed.

These results established the identity of the virus that caused yellow flecking on rose leaves in India as SLRSV. To our knowledge, this is the first report of SLRSV infecting rose in India."