Title: Marker Assisted Background Selection for the Introgression of Black Spot Resistance into Cultivated Roses
Authors: T. Debener ; B. v. Malek ; M. Schreiber ; R. Drewes-Alvarez
Authors affiliation: Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding, Ahrensburg, Germany,University of Zurich, Institute of Plant Biology, Zurich, Switzerland; HTW-Dresden (FH), University of Applied Sciences, Dresden, Germany
Published in: Europen Journal of Horticultural Science, Volumn 68, pages ??, (2003).
Abstract: “In a model breeding program Rdr1 , a gene conferring resistance to black spot ( Diplocarpon rosae Wolf ) was introduced from a wild rose species into the genetic background of cultivated tetraploid roses. The chromosomal set of the original diploid donor genotype, a Rosa multiflora hybrid, was doubled via colchicine treatment and crossed to a tetraploid hybrid tea variety. The resulting hybrid genotype, 91/100-5, was crossed to a Floribunda garden rose and the resulting progeny was tested for black spot resistance. AFLP markers were then used to screen a subset of the resistant progeny for the fraction of donor genome, which segregates from 32 to 84 % in this population. The genotype with the smallest number of donor-specific markers was further backcrossed to the Floribunda parent and the hybrid tea grandparent, and the analyses conducted in the previous generation were repeated. The BC2population segregated for the fraction of the original donor genome, ranging from 12.3 % to 32 %. A parallel screen for morphological markers in the BC1demonstrated the superiority of the molecular markers for the reduction of the genetic background of wild rose species in introgression programs allowing a more efficient utilization of wild rose germplasm.”
http://www.ulmer.de/scripts/artikeluc.dll?AID=15772&MID=1433&TIX=0&AROOT=2178&UID=C6FAB9A21DE354DB94A3A7656AD0BFAF44A1B3D3
Link: www.ulmer.de/scripts/artikeluc.dll?AID=15772&MID=1433&TIX=0&AROOT=2178&UID=C6FAB9A21DE354DB94A3A7656AD0BFAF44A1B3D3
Do we want to?
where’s the fun in that approach, its like painting by numbers.
Im happy to let chance play its part.
I would have to agree. Plant breeding is half art and half science.
Lets hope that they do not patent the genes. Then we can use their genes to “fine tune” better plants for our local climates.
wouldn’t worry, its not like we can stop “thier genes” from turning up in OP seed.
Doesn’t blackspot mutate?
Jinks said: “wouldn’t worry, its not like we can stop “thier genes” from turning up in OP seed.”
You have every right to be worried. If the company decides to patent the genes and “go Monsanto” on us, they could sue people who make use of plants that contain the gene via chance open pollination. Haven’t you heard about Monsanto’s successful lawsuits against people like that Saskatchewan farmer who accidentally got one of their patented genes in his crops through airborne pollination?
Link: www.tv.cbc.ca/national/pgminfo/canola/
I knew there was a reason I never bought my concentrate from Round Up.
That is truely scary. Gee, I wonder if they will own the children of people who get their DNA genetically altered to prevent diseases in the future?
I think that this is a great approach. One can select advanced generations for the presence of a gene of interest and out of those select for individuals which have less of other genes that came along from the “wild” type parent supplying the gene of interest. This is especially valuable for agronomic crops where additional traits coming along can be very problematic in for instance the case of wheat and breadmaking if additional genes throw off protein profiles and such.
This approach does not have anything to do with placing foreign genes into a rose, although that can be done too. All they did was make sexual crosses, like we do, but through molecular markers follow the transmission of the disease resistance gene of interest through the generations and then select for individuals with the gene and with as little additional genetic contribution from the R. mult. parent as possible. Yes, it is just one blackspot resistance gene and there’ll probably be races that will overcome it shortly if that hasn’t already happened already. Yes, having some additional “wild” rose contribution may be desirable and not no necessary to weed out as with other crops. What a great step forward for plant breeding, however, and honor that this work has been done in roses. Roses have been chosen as one of the early “model” crops where this work has been done due to the forward thinking of these researchers. Too bad I heard due to cutbacks in the German government Debener’s position has been phased out. He was (still is in my opinion) the world’s leading rose molecular geneticist. Does anyone have an update on him and the program?
Sincerely,
David