Julie, I have not seen your experimental details so I am only guessing at the possibilities that I am here presenting.
Your statement: âAs the plates were moved to germination conditions, the composting resumed and the heat generated was high enough to induce a secondary dormancyâ seems to indicate that you used a different type of compost activator than used in Morpethâs Ph. D. thesis. What were the components of your compost activator? Also, did you use a germinating medium that the compost activator could react with such as peat? Also, did your compost activator contain digestive enzymes or microbes?
Their germinating medium and activator were:
" Seeds were mixed with vermiculite, an inorganic medium with good water retention properties, since it is essential to keep the seeds moist during temperature pretreatment (Gordon and Rowe, 1982; Gosling and Aldhous, 1994). Vermiculite contains no nutrients and was sterilized (autoclaved) before use.
Seed samples were subjected to different pretreatment conditions (Fig. 1). The commercial (control) treatment was based on moist vermiculite and seed (10:1 w/w vermiculite/seed). The enhanced treatment was achieved by the addition of GarottaâM (J. Arthur Bowerâs, Lincoln, UK), a commercial compost activator (10% w/w GarottaT"'/seed). This activator is a widely available sterile product consisting of a mixture of inorganic mineral nutrients such as calcium carbonate, ammonium salts and phosphates (Morpeth, 1998). Any visual changes in the physical properties of the seeds were recorded on a weekly basis. The control and enhanced treatments were applied to fresh collections of seeds, from the same stock bushes, every year from 1992 to 1996. Detailed data are provided for the 1996 experiments."
They depended on the natural âbugsâ found on the seed to eat the seed coat (when they used sterilized seeds, the compost activator did not work).
I âsuspectâ that you either used a degradable germinating medium or a compost activator that contained active enzymes and/or microbes.
You mentioned that you also did enzyme experiments. Please give more detail on your enzyme treatment experiments as I was able to get results similar to what Yambe et.al. reported ( http://home.neo.rr.com/kuska/enzymeuse.htm , please follow the links from that link).
The following is another enzyme treatment paper:
Title: Coffee (coffea arabica L.) seed germination after treatment with different concentrations and embedding times in cellulase.
Authors: Sales, Juliana De Fatima; De Alvarenga, Amauri Alves; De Oliveira, Joao; Nogueira, Francisco Dias; Rezende, Lucio Costa; Silva, Fabiano Guimaraes.
Authoes affiliation: Fisiologia Vegetal-UNIVERSIDADE FEDERAL DE LAVRAS/UFLA, Lavras, Brazil.
Published in: Ciencia e Agrotecnologia , volumn 27, pages 557-564, 2003).
Abstract: âThe seeds of coffee trees germinate slowly, because the seeding is done soon after the crop is harvested, which coincides with the cold period of the year, and also due to the parchment, which constitutes a barrier that hinders the absorption of water for the seed. Aiming to evaluate the effect of exogenous application of cellulase enzyme to coffee seed germination, an expt. was performed soaking coffee seeds, cultivar âAcaia do Cen-adoâ. in cellulase soln. at different conchs. (0, 1.6, 3.2, and 6.4, g L-1) in 0.05 M K-citrate buffer at pH 4.8 for 0.72 and 144 h. It was found that the cellulase enzyme at the concn. of 6.4 g L-1 provided the greatest index of speed germination and the greatest germination percentage when the seeds remained in contact with the soln. for 144 h.â