The following was stated: “It’s interesting to note the 16 cases where ploidy had been determined previously by microscopy, and that in 6/16 of them, the ploidy is now disputed with the flow cytometry method. 6/16 is quite a high proportion.
What is your take on that, @henry_kuska ? Is the cytometry result the “definitive” one?”
H. Kuska comment: I did not interpret this paper to indicate that microscope results were not definitive. The present authors used the microscope method to confirm their cytometry results for their sample.
I did extensive microscope - pollen work and found some/many ? examples of samples with more than one size pollen. example - Rugelda X R15 is blooming, Pollen available
Is the pollen size dependent on external conditions such as temperature, sunlight, soil conditions, first hips of the season, later hips, etc.?
effect of soil nitrogen on pollen production - Google Scholar