Title: Colchicine-Induced Tetraploid and Cytochimeral Roses
Authors: Peter Semeniuk; Toru Arisumi
Published in: Botanical Gazette, volumn 129,pages 190-193, (1968).
Abstract: “A complete tetraploid, a 4-2-2 periclinal chimera, and a normal diploid shoot were isolated from a colchicine-treated diploid rose shoot. The ploidies of the three histogenic layers of the shoot apex were confirmed by the size of stomata and pollen grains and by chromosome number from roots. The experiment showed that colchicine had penetrated into the histogenic layers of the shoot apex in only a very small percentage of the treated axillary buds.”