The colour of the flower as well as the central golden stamen display do hint of an Altissimo parentage, I think?
I hope to have 4 seedlings in about 8-14 days time
George, how are you ‘culturing’ the embryos once you extract them?
Hi Don.
I clean out a jar (eg. baby food jar), and then stand it upside down to get the water droplets to all roll out. Then I blow into it some exhaled breath to get a little fogging happening, and then place the embryos inside, and seal the jar with its lid. I keep the jar indoors in a well lit room away from direct sunlight. Every day I open the lid and refresh the air by blowing into it gently.
It’s cool to be able to actually observe the transformation into a rooted seedling just beside the computer station!!
The picture below shows a close up of one of the above four sibbling embryos, now at day 2…You can see the cotyledons are starting to spread apart.
All the four babies from this hip of the red climber are doing well, and they should all be in soil in 2 weeks happily enjoying their new life in this world.
George.
George, if you send me your email address I’ll forward you some potentially useful information on culturing your embryos.
All the Best
Don
Email address sent to you Don.
George
Don, I have read the material you sent. I just sent you a follow-up email regarding this, using the direct “email” function here.
George.
For those that are interested, here are the embryos today- day 4 of their life in the jar!
Notice the one on the bottom left is a tricotyledon.
No palm readings please!
“No palm readings please!”
Your life line has a lot of green in its future…
George, how do you remove the embryos from the achenes? I had trouble removing them without damaging them.
Hi again all…
Just for fun, here is the fastest of the four embryos (now day 5)…
I am almost tempted to plant this one today, but I think I will give it another 24 hrs, and plant it tomorrow.
Have a nice day/night all…
George.
How many days did it take for you to get those seedlings from those embryos?
Enrique, so far it has been 5 days (read above history)… Three of the four embryos shown in this thread have now developed green leaves and rootlets, but one of them has yet to go fully green and is very slow to change…It too should start to germinate soon, as I can just see a hint of green coming onto it as I look at it right now.
Wow.
I guess it is really easy then.
I will purchase the supplies tomorrow online. I have several OP seeds of my kordesii X basye’s Amphidiploid seedling (it has very large easy to handle seeds.)
Enrique, if this is your first attempt at extractions, practice on seeds you don’t care about until your strike rate approaches 90% …embryo-ok-in-seed/embryo-ok-out-of-seed.
This type of result took me more than 100 attempts to acheive I am sure… It is not that easy!
George.
Day 6 of embryo ‘culture’…
The tricotyledon at 3 o’clock is not quite ready to plant, but the 5 o’clock and 9 o’clock seedlings are going into seed raising mix now. I will plant them with the cotyledons exposed to the light and air.
The 12 o’clock embryo has a very dense type of leaf structure all wrapped up like a cabbage into a tight conical form. The small hook-like extension towards its top right is the growing radicle…it will be interesting to see its final morphology. Sometimes, the cotyledons are so large that they are actually folded onto themselves to maximise surface area to volume.
So what kind of tools do you use to crack the seed coat and what tool do you use to separate the embryo from the seed coat? Do you stratify the seeds first or do you do this after they are removed from the hips?
Adam,
I use a very sharp box cutter type knife, my two bare hands and a cutting board from the kitchen. Seed is freshly picked off the bush, and immediately cultured after extraction. Stratification is not reqired at all.
Disclaimer…Anyone that wishes to try this idea of mine assumes their own personal responsibility for finger cuts and other personal injuries which may occur.
George,
I can’t begin to express how much I’ve enjoyed watching these seedlings come along. It’s been a joy.
For us in North America, there’s a product offered by Lee Valley tools that’s a finger wrap that can’t be cut into. It’s both reusable and it does work (I’ve used it when I was doing woodworking and unsure of myself with a new carving tool.) I will wrap my endangered fingertips before I try some rescues.
Link: www.leevalley.com/wood/page.aspx?c=2&p=31213&cat=1,42207
Hi Ann.
These two slow-coaches are typical of what I mentioned earlier about some embryos being a little recalcitrant to throw a rootlet from their developing radicle/stem.
You can see the tricotyledon embryo very clearly in this picture. The other sibbling in this picture seems to be expending lots of its energy in trying to unfold the tightly folded cotyledons…Maybe they are going to be BIG…
Their two sibbling which were potted up a few days ago are doing ok.
Take care all,
George.
George, your posts on this thread have me dying from cuteness overload =/
Good work