"Abstract
The complete genomic sequence of rose yellow mosaic virus (RoYMV) was determined and found to have all the features that are characteristic of members of the family Potyviridae. The RoYMV genome is 9508 nucleotides long excluding the 3?-poly-(A) tail and contains a single open reading frame encoding a polyprotein of 3067 amino acids. The RoYMV P3 and CI cistrons are shorter than those of other members of the family Potyviridae, and the 6K1 cistron is completely absent. Comparative sequence analysis revealed that RoYMV had highest amino acid sequence identity across the entire genome sequence to brome streak mosaic virus (33 %) and to turnip mosaic virus (30 %) at the coat protein level. Based on its low sequence similarity to known members of the family Potyviridae and phylogenetic analysis, RoYMV appears to be a distinct, previously undescribed, member of this family."
See: Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae | SpringerLink
When I first found this paper today, it stated that the full copy was available, but when I clicked the link it did not come up. The link now works:
http://link.springer.com/content/pdf/10.1007%2Fs00705-013-1686-7
Of particular interest to me is the discussion about the possible mode of transmission:
" None of the KITC, IGN, CCC, and PTK motifs [11, 37] are present in the RoYMV HCPro. Instead, a putative C-2x-C eriophyid mite transmission
motif [39] is found at amino acid residues 48-51 at the N-terminus of the protein, suggesting that this virus may possibly be transmitted by eriophyid mites."
I received an e-mail that the second link that I sent only gave the first 2 pages. I went to my wife’s computer and yes only the first 2 pages came up. Try this link:
If that also will not work, the following discussion is worth pointing out:
“As mentioned above the RoYMV HC-Pro is missing the aphid-transmission motifs. Similarly, the DAG aphid transmission motif, found at the N-terminus of the CP peptide [20], is missing from the CP of RoYMV. Both HC-Pro and CP mediate aphid transmission of members of the genus Potyvirus. While, as discussed above, the other peptides have similarities to viruses from almost all of the genera of the family Potyviridae, the HC-Pro and the CP amino acid sequences were determined by BLASTP analyses to be similar only to those of members of the genus Potyvirus, which are known to be aphid transmitted [9]. Rose cultivars are clonally propagated [16]. It is possible that RoYMV may have originally been transmitted by aphids and that aphid transmission was lost during an extended period of continuous vegetative propagation.”
Hi Henry!!
We just received the proofs from the journal late last week!! They are fast in getting it up and you are amazing Henry in finding rose articles so quickly. Thank you for posting the abstract and spreading the word. We have a separate paper coming out in the near future that focuses on the symptoms found on different roses and some aspects of transmission. It is interesting that this and other of the newer viruses that are being characterized are relatively widespread in public gardens (typically these characterizations start with sick plants people send us from public gardens). Roses are coming from different sources to these gardens and many roses are in somewhat close proximity.
Well this morning the two links (that worked for me yesterday on my computer) today only give the first 2 pages. Fortunately I had saved the manuscript yesterday.
The following from the full paper was also of interest to me:
“The common rose viruses apple mosaic virus (ApMV) (synonym rose mosaic virus [RMV]) and prunus necrotic ringspot virus (PNRSV) usually express symptoms early in the season, and the plants become asymptomatic later, with no possibility to detect these viruses. In contrast, the symptoms caused by RoYMV [19] persists throughout the season, and the virus can be detected. One possible explanation could be the suppression of posttranscriptional gene silencing [37], which is reported to be manifested by the P1 [33, 40] or HC-Pro [4, 30] peptides from members of the family Potyviridae.”
H.Kuska comment. Thus, the temperature dependence that has been the unappreciated “monkey wrench” in some of the earlier rose virus transmission studies that were done in warm climates does not complicate the behavior here. See: http://home.roadrunner.com/~kuska/high_temperature_effect_on_pnrsv.htm