Pollen treatment for mutation induction

Does anyone have access to the full paper for the important experimental details such as: chemical used, how applied, duration, etc.?

Title: Pollen treatment for mutation induction in Eucalyptus globulus ssp. globulus (Myrtaceae)

Authors: L. J. McManus A , J. Sasse B , C. K. Blomstedt A , C and G. Bossinger A , D

Authors affiliation: A The School of Forest and Ecosystem Science, University of Melbourne, Creswick, Vic. 3363, Australia.

B Sassafras Group, 2 O

I received a copy of the paper. Unfortunately, the mutant used is ethyl methanesulfonate (EMS) in paraffin oil.

I do not recommend that the amateur rose hybridizer try utilizing this “stuff”. Perhaps the following web page will convince you not to try it.

http://www.agron.missouri.edu/mnl/74/12kato.html

However, the use of pollen in paraffin oil with or without a different chemical added; may be of interest to those who wish to try something different. The authors did run a comparison of normal pollination versus using pollen in just paraffin oil. 27.2 % of the fruit aborted using normal pollination, and 37.3 % aborted when the pollen was applied in paraffin oil. Although there were less successful pollinations, the amount of successful pollinations produced using the oil technique was still within the useful range. In previous experiments I tried to store pollen in organic solvents in order to extend the lifetime of the pollen.

See (for example):

http://www.rosehybridizers.org/forum/message.php?topid=1757#1771

I was mainly interested in organic solvents that would evaporate quickly or had some water solubility as I assumed that the germination would not start until the solvent evaporated or was diluted by the stigma fluids. This paper suggests that I should try storing in paraffin oil over the winter and then pollinating directly with the mixture (instead of washing the oil away with acetone).

I also wonder if I will get any mutations/ other useful effects treating pollen with Preem/paraffin oil mixtures or hormones like 2,4-D

(see:

http://www.rosehybridizers.org/forum/message.php?topid=3807#3807

and

http://www.rosehybridizers.org/forum/message.php?topid=3021#3097

)