I’ve tried surflan, and as many you know, I may have success with Rosarie d’lhay, but it hasn’t bloomed since the two years I’ve applied it. I’m thinking this is something that needs to be grafted…
This year I have obtained 500 milligrams of Colchicine, and bought all necessary supplies to keep me and the family safe. I have a mini refrigerator, new and sterilized doppler bottle, eye glasses, etc… Let’s just say everything is ready for Spring. I’ve fixed the door knobs of the backyard, so that when I do apply colchicine, the gates will be locked so that the kids won’t go in the backyard and potentially rub up against stuff.
Now I am going to germinate seeds from my openly pollinated R. foliolosa. Looking the amount I’ve cleaned, it is easily over a thousand seeds. I am thinking that obviously there will be many selfs as there maybe many hybrids. And hopefully when a few do germinate, I will apply the 5 percent solution of colchicine to many of the selved seedlings.
I will be using Dr. Basye’s method as described in his 1990 article in the ARS, using only half a drop overnight (I’m going to say around 8 hours). However, I am very intrested in trying to make Nigel Hawthorn fertile, a hybrid between a rugosa and hulmutha. It’s very sterile, but the problem I am seeing is how to maintain colchicine the buds long enough before it evaporates. Last Spring I applied a DMSO/surflan mixture without any success. Although I think I may had made too strong of a mixture…
Since I am not using DMSO, my thinking is probably using a cotton, or even using flavorless jello or algar. Has anybody else heard of any other methods?
In another experiment, I will be using the Autumn Crocus bulb, from which colchicine is made. During my research, it seems that many Marijuana users also attempt to make Marijuana polyploids. The most common method I heard was to mash a corm of the Autumn Crocus and mix it with an equal amount of water. After a careful extraction with a coffee filter, seeds from Marijuana are then submerged into the solution for many hours and then planted. Most of the survivors are usually polyploids.
Now I am thinking of using this method, but only with seeds of R. foliolosa. However the seed coat may present some trouble, and I’m thinking of stratifying them first to weaken, and to nick a few with sand paper, just like a gardener does with Morning Glories. But the fact the seeds of R. foliolosa all float presents difficulties, but perhaps I can fasten it to a tea bag or something…